In vitro bone metastasis dwelling in a 3D bioengineered niche

Bone is the most frequent metastasis site for breast cancer. As well as dramatically increasing disease burden, bone metastases are also an indicator of poor prognosis. One main challenges in investigating cancer engineering vitro models that replicate features vivo environments. Such ideally enable biology metastatic cells to mimic their behavior closely possible. Here, taking benefit cutting-edge technologies both microfabrication and cell biology, we have developed bone-metastasis model. To do so first 3D printed a scaffold reproduces trabecular architecture can be conditioned with osteoblast-like cells, collagen matrix, mineralized calcium. We thus demonstrated this device offers adequate soil seed primary cells. In particular, patient-derived xenografts being considered better approach than lines achieve clinically relevant results, demonstrate ability biomimetic niche model host xenografted These xenograft show long-term survival maintain cycling propensity, exhibit same modulated drug response vivo. This experimental system enables access idiosyncratic microenvironment metastasis, which has implications testing.